|
KMID : 0665420200350060597
|
|
Korean Journal of Food Culture
2020 Volume.35 No. 6 p.597 ~ p.604
|
|
|
Antioxidant and Hepatoprotective Activities of Bitter Melon (Momordica charantia Linn.) Leaves against Oxidative Stress
|
|
Jeon Ah-Yeong
Cheon Won-Young
Yoon Ji-Min
Kim Dae-Jung
Kim Young-Hwa
|
|
|
Abstract
|
|
|
|
This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantiaLinn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g,respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations ofethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mgcathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed thehighest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH)free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. TheE100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extractshowed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactiveoxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.
|
|
|
KEYWORD
|
|
|
Bitter melon leaf, radical scavenging activity, hepatoprotective effect, intracellular reactive oxygen species, antioxidant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|